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Effect of Erl combined with TC-HT on <t>p-EGFR</t> protein expression in A549 cells. Western blotting of p-EGFR protein expression in A549 cells treated with 10 µM Erl, moderate temperature TC-HT, and the combination treatment. The expression levels of p-EGFR were normalized to GAPDH. Each relative expression level was compared with the control and represented as fold relative to the control. Data are shown as mean ± standard deviation (n=3). Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test. **P<0.01, ***P<0.001. Erl, erlotinib; TC-HT, thermal cycling-hyperthermia; p, <t>phosphorylated;</t> t, total; ctrl, control.
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Effect of Erl combined with TC-HT on p-EGFR protein expression in A549 cells. Western blotting of p-EGFR protein expression in A549 cells treated with 10 µM Erl, moderate temperature TC-HT, and the combination treatment. The expression levels of p-EGFR were normalized to GAPDH. Each relative expression level was compared with the control and represented as fold relative to the control. Data are shown as mean ± standard deviation (n=3). Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test. **P<0.01, ***P<0.001. Erl, erlotinib; TC-HT, thermal cycling-hyperthermia; p, phosphorylated; t, total; ctrl, control.

Journal: Oncology Reports

Article Title: Thermal cycling?hyperthermia sensitizes non?small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib

doi: 10.3892/or.2025.8891

Figure Lengend Snippet: Effect of Erl combined with TC-HT on p-EGFR protein expression in A549 cells. Western blotting of p-EGFR protein expression in A549 cells treated with 10 µM Erl, moderate temperature TC-HT, and the combination treatment. The expression levels of p-EGFR were normalized to GAPDH. Each relative expression level was compared with the control and represented as fold relative to the control. Data are shown as mean ± standard deviation (n=3). Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test. **P<0.01, ***P<0.001. Erl, erlotinib; TC-HT, thermal cycling-hyperthermia; p, phosphorylated; t, total; ctrl, control.

Article Snippet: The specific primary antibodies against phosphorylated EGFR (p-EGFR; cat. no. 4407; Cell Signaling Technology, Inc.), poly ADP-ribose polymerase (PARP; cat. no. 9542; Cell Signaling Technology, Inc.), p-JNK (cat. no. 4668; Cell Signaling Technology, Inc.), p-Akt (cat. no. 4060; Cell Signaling Technology, Inc.), total Akt (t-Akt; cat. no. 9272; Cell Signaling Technology, Inc.), MutT homolog 1 (MTH1; cat. no. 43918; Cell Signaling Technology, Inc.), p-p38 (cat. no. GTX133460; GeneTex, Inc.), Cdc2 (cat. no. GTX108120; GeneTex, Inc.) and GAPDH (cat. no. GTX100118; GeneTex, Inc.) were used.

Techniques: Expressing, Western Blot, Control, Standard Deviation